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Orientation of swimming Euglena and Astasia in the field center, differentiated in vertical (0°/180°±22.5°, black), horizontal (90°/270°±22.5°, white), and diagonal (45°/135°/225°/315°±22.5°, gray) direction. Each column in represents>2300 (Euglena) and >50 (Astasia) cells. Notice the corresponding shift from vertically aligned mobile wild-type Euglena in contrast to the chloroplast-free Astasia.
