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The influence of the competitive antagonist, MRS1220, and/or the allosteric ligand, VUF5645, on the dissociation kinetics of the fluorescent adenosine derivative, ABA-X-BY630. ABA-X-BY630 (30 nM) dissociation in the absence and presence of a 1 μM MRS1220, 1 μM VUF5645, or 1 μM MRS1220 and 1 μM VUF5645. Representative data performed in duplicate; each replicate represent the average fluorescence at the plasma membrane of 10 individual cells.
