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. 2014 Feb 27;171(5):1250–1259. doi: 10.1111/bph.12528

Figure 3.

Figure 3

ORAI1-3 currents inhibited by 4-CEP, 4-CmC and 4-ClP in STIM1/ORAIs cells. Whole-cell currents were recorded in the HEK293 T-REx cells co-expressing STIM1-EYFP and ORAI1-3, or expressing ORAI3 alone. The store-operated currents in STIM1/ ORAI1-3 cells were evoked by TG (1 μM) in the pipette solution, and the current in ORAI3 cells was induced by 2-APB (50 μM). The time course for the amplitude of whole-cell currents was given at ±80 mV with corresponding leak-subtracted I/V relationships before and after treatment (inset). (A–C) Effect of 4-CEP (500 μM) on ORAI1-3 currents evoked by TG (1 μM). (D–F) Effect of 4-CmC (500 μM) on ORAI1-3 currents. (G–I) Effect of 4-ClP (500 μM). (J) Effect of 4-CEP on the non-transfected HEK293 T-REx cells. (K) ORAI3 current induced by 2-APB and the effects of 4-ClP, 4-CmC and 4-CEP (500 μM). (L) % inhibition by 4-ClP, 4-CmC and 4-CEP of the whole-cell currents measured at −80 mV in the STIM1/ORAI1 (S1O1), STIM1/ORAI2 (S1O2), and STIM1/ORAI3 (S1O3) (with TG); and ORAI3 (with 2-APB) cells (n = 5–10 in each group).