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. 2014 Feb 27;171(5):1250–1259. doi: 10.1111/bph.12528

Figure 4.

Figure 4

Extracellular effect of 4-CEP on ORAI3 channels. (A) Example of ORAI3 currents inhibited by 4-CEP (500 μM) in the STIM1/ORAI3 cells using outside-out patch configuration. (B) The I/V curves for (A). (C) Mean ± SEM for the current amplitude of outside-out patches (n = 3). (D) Intracellular application of 500 μM 4-CEP in pipette solution did not block the activation of ORAI3 by 2-APB, but extracellular application of 4-CEP (500 μM) significantly inhibited the ORAI3 current. (E) The I/V curves for (D). (F) Mean ± SEM for the amplitude of whole-cell current recorded with intracellular 4-CEP (500 μM) (in) and outside bath perfusion with 4-CEP (out) (n = 3). * P < 0.05, ** P < 0.01 and *** P < 0.001.