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. 2014 Mar 13;10(3):e1004187. doi: 10.1371/journal.pgen.1004187

Figure 4. Time lapse microscopy of chromatin dynamics during resection.

Figure 4

2D stacks (50×200 ms frames) of the ParB2-GFP focus were acquired in wt and yku strains in which the ParB1-mCherry spot was lost, starting at 8 minutes post-addition of galactose to the medium. A. Mean-square displacement (MSD) for wt (n = 12) and yku (n = 3) cells of the uncleaved locus (black line) and at the indicated times after HO induction. B. Diffusion coefficients (D) for wt (black) and yku (red) calculated from the slope of the first 2 sec of the MSD shown in A and normalized to the average D = 1.9×10-2 µm2/sec for uncut DNA set at 100%.