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. 2014 Mar 13;10(3):e1004200. doi: 10.1371/journal.pgen.1004200

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© 2014 Eliazer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) A Lsd1 binding peak is observed in the engrailed promoter in escort cells. (B) RT-qPCR of whole ovary extracts from bam^Δ86 and bam^Δ86 Lsd1^ΔN double mutants. The bam^Δ86 Lsd1^ΔN double mutant samples exhibit a relative 6-fold increase of engrailed transcripts when compared to bam^Δ86 mutants. (C and C′) w¹¹¹⁸ and (D–E′) Lsd1^ΔN homozygous germaria stained for Engrailed (En) (green) and VASA (red) and DNA (blue). Arrows point to Engrailed positive cells. In w¹¹¹⁸ control germaria, Engrailed is expressed in the terminal filament and cap cells, whereas Lsd1 mutants display engrailed expression in the anterior escort cells. Scale bars = 10 µM.