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Proximal signaling through major hubs is not affected by ShcD expression. (A) The consequence of ShcD-GFP on signaling events occurring before and after stimulation with 50 ng/ml EGF (10 min) was monitored by immunoblotting lysates for common downstream effectors to compare total protein levels with the phosphorylated fraction. (B) Similarly, cells transfected with ShcD-FLAG were assayed for phosphorylation of FAK on two distinct residues.
