FIGURE 6:

ShcD promotes EGFR-dependent cellular migration. (A) To determine the physiological consequences of ShcD-induced EGFR phosphorylation, confluent monolayers of transfected cells were serum starved and treated with DMSO (vehicle) or 1 μM AG 1478 6 h before inflicting scratch wounds. Matched regions of interest were imaged 0, 18, and 48 h after the procedure. Scale bar, 140 μm. (B) Wound closure was measured by T-Scratch software and expressed as Δ area. Averages of three biological replicates per condition are shown and were compared by one-way ANOVA (p = 0.0038), followed by Bonferroni's multiple comparison test. *Significant difference. Error bars denote SEM. (C) Levels of cellular apoptosis inferred from PARP cleavage patterns in suspension and adherent cells after serum deprivation and 10 min of EGF stimulation.