FIGURE 2:

Kinetics of ρ⁻/ρ⁰ cell production. The Tet-γ, Tet-δ, and Tet-ε strains and their parental strain SDC22 (WT) were grown in rich galactose in the presence of 10 μM doxycycline. The cultures were refreshed several times with the same medium to produce a total of 40 generations, which was estimated by measuring the turbidity at 650 nm. The contents in ρ⁻/ρ⁰ cells were determined at the indicated number of generations.