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. 2014 Mar 15;25(6):811–827. doi: 10.1091/mbc.E13-08-0443

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© 2014 Rosado et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Localization of DAAM and FMNL formin subfamily members to sarcomeres. (A) Immunohistochemical localization of the formins (green) DAAM1, FMNL1, FMNL2, and FMNL3 in relation to Z-bands (red, α-actinin) in neonatal primary mouse cardiomyocytes cultured 11 d in vitro. White boxes on cell overview (first row) indicate the zoom-in regions (rows 2–4). Graphs below depict fluorescence intensity in the two channels over 12 μm. Daam1 signal colocalized with α-actinin labeling of the Z-band. FMNL1 signal appeared as a broad band surrounding the α-actinin–labeled Z-band, with peaks on either side of the Z-band (white arrows in zoom; black arrows in graph). FMNL2 signal filled the area between α-actinin–labeled Z-bands. FMNL3 signal appeared as a few puncta with no regular sarcomere-related pattern. (B) Immunohistochemical localization of formin (green) and α-actinin (red) for DAAM1, FMNL1, and FMNL2 in mature myofibrils isolated from 2-mo-old mice. All three formins colocalize with α-actinin, indicating robust sarcomere localization. Scale bars, 10 μm.