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. 2014 Mar 15;25(6):841–851. doi: 10.1091/mbc.E13-09-0543

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© 2014 Liu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Borg5 regulates persistent directional migration of the cardiac ECs. (A) Heart explant angiogenesis assay. Wild-type and Borg5-null heart explants were stained with isolectin β4 to label ECs after 6 d of culture. Three-dimensional images were obtained by confocal microscopy and reconstructed in Imaris. Regions in the white dashed squares were enlarged to the right of each image for better visualization. Scale bars: 0.5 mm. (B) Quantification of sprout length. (C) Examples of automated tracking of EC migration from heart explants during day 4 to day 5 of culture. EC nuclei were labeled with SYTO-16 green fluorescent dye. Tracked nuclei are highlighted in white, and individual tracks are displayed as time color-coded lines. Time is expressed as h:min:s. Scale bar: 100 μm. (D) An illustration of individual cell displacement and path. (E) Quantification of persistent directional migration (straightness, displacement/path). (F) Quantification of migration speed (path/time). Error bars: SEM. Statistical analysis was done by two-tailed Student's t test.