FIGURE 5:

Reduction of H3K9me3/S10ph is associated with in increased level of paused RNAPIIS5ph at repressed genes in differentiated C3H cells. (A) Chromatin from differentiated C3H cells was precipitated with antibodies that recognize H3K9me3/S10ph, H3K27me3, Ezh1, Ezh2, and RNAPIIS5ph and hybridized to an Agilent tiling oligonucleotide microarray covering the 2-Mb region of mouse chromosome 3 described in Figure 4. Peaks of enrichment are indicated by orange squares. Blue lines are alignment lines. Regions containing repressed genes are indicted by dashed boxes. (B) Effect of H89-induced reduction of H3K9me3/S10ph at repressed genes on binding of RNAPIIS5ph in differentiated C3H mesenchymal cells. The y-axis shows the ratio of enrichment of RNAPIIS5ph at the promoters of the indicated genes in H89- treated cells relative to enrichment cells treated with vehicle (dimethyl sulfoxide). Bars show mean ± SD. n = 5 biological replicates. Statistical significance was assessed by paired t test.