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. 2014 Mar 15;25(6):948–964. doi: 10.1091/mbc.E13-09-0523

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© 2014 Arndt et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Immunoelectron microscopic analysis of TPC1 in adult mouse testis. Ultrathin sections of adult mouse testis of wild-type (left, TPC1 [+/+]) and TPC1-knockout males (right, TPC1, [−/−]) were labeled with anti-TPC1NK antibody, followed by incubation with goat anti-rabbit IgG conjugated to colloidal gold particles. TPC1-null testis tissue and the midpiece (mp) and proximal part of the principal piece (pp) of sperm tails of wild-type males did not show any precipitated immunogold particles. However, in wild-type spermatides, immunogold particles were only detected at a narrow area between the plasma membrane (pm) and the subacrosomal space (ss). This area holds membranes lining the acrosome. Although these membranes were only poorly preserved due to the fixation and embedding procedure, gold particles were found to follow the contour of the outer acrosome membrane. Images in the second line show different tissue areas with similar gold particle distribution. nm, nuclear membrane; nu, nucleus.