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. 2014 Jan 17;3(3):334–345. doi: 10.5966/sctm.2013-0129

Figure 2.

Figure 2.

Bulge explant culture and ex vivo expansion. (A): Time course of ex vivo expansion of canine epidermal neural crest stem cells (cEPI-NCSC) from a primary hair follicle (dog 4: phase contrast optics). Scale bars = 400 μm. (B): cEPI-NCSC short-term ex vivo expansion, representative image. Cells start to divide less than 24 hours after plating. Millions of cells are obtained from one bulge explant by day 5 of ex vivo expansion (dog 3: phase contrast optics). Scale bars = 200 μm. (C): cEPI-NCSC growth curve during ex vivo expansion. On average, 3 million cells were obtained per hair follicle within 11 days after explantation.