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. 2014 Jan 17;3(3):334–345. doi: 10.5966/sctm.2013-0129

Figure 5.

Figure 5.

Characterization of canine epidermal neural crest stem cells (NCSC) by immunocytochemistry. Representative images. Canine epidermal NCSC express at the protein level NCSC signature genes, general neural crest markers, and a general stem cell marker. Images shown are the antibody-specific (black and white) and merged images (color) using DyLight 488- or DyLight 594-conjugated secondary antibodies. Blue, DAPI nuclear stain. (A, A′): Transcription factor Ets1 shows mainly nuclear immunoreactivity. (B, B′): Thop1 shows variable cytoplasmic immunoreactivity. (C, C′): Transcription factor Msx2 with mostly cytoplasmic immunoreactivity. (D, D′): Cytosolic protein Crmp1 has variable cytoplasmic expression. (E, E′): Ube4b shows strong cytoplasmic expression. (F, F′): Myo10 shows strong cytoplasmic immunoreactivity. (G, G′): Adam12, a membrane-anchored protein, shows variable cytoplasmic expression. (H, H′): Cryab has diffuse cytoplasmic immunoreactivity. (I, I′): The intermediate filament Nes shows cytoplasmic immunoreactivity. (J, J′): Sox10, a nucleo-cytoplasmic shuttle protein, shows mainly cytoplasmic immunoreactivity. Scale bars = 50 μm. Abbreviation: DAPI, 4′,6-diamidino-2-phenylindole.