Skip to main content
. 2014 Mar 13;10(3):e1004232. doi: 10.1371/journal.pgen.1004232

Figure 5. High levels of un-cleaved MYB33 transcripts remain strongly repressed.

Figure 5

(A) Aerial view of 29-day-old rosettes from MYB33 and mMYB33 transgenic plants (myb33 genotype) grown under short day conditions. Scale bar = 10 mm. (B) Alignments between miR159a and the wild type and mutated miR159 target sites in MYB33 and mMYB33 constructs used to transform Arabidopsis. Nucleotide changes made in mMYB33 are highlighted in red. Both MYB33 and mMYB33 encode the same protein. (C) Average rosette sizes of 29-day-old T2 transgenic plants from MYB33 lines grown side by side with Col-0 and myb33. Unit of measurement shown is relative pixel. Multiple plants for each line were grown on soil side by side with Col-0 and myb33. More than fifteen transgenic plants in each line were analysed. Error bars represent SD. (D) The steady state mRNA levels of un-cleaved MYB33 in the rosettes of multiple MYB33 (lines 1–6) and mMYB33 (lines 1–2) T2 transgenic lines. (E) Relative mRNA levels of CP1 in the rosettes of the same MYB33 and mMYB33 lines. All mRNA levels were normalized with CYCLOPHILIN 5. All measurements are the average of three technical replicates with error bars representing the SEM.