Table 1.

DNA	Length/bp	Sequence*	Binding affinity**
Ori	67	5′-AAAATTTAAAAGAAGACACCAAATCAGATGCCGCCGGTCGCC GCCGGTAGGCGGGACTTCCGGTACA -3′	++++
NSBE1+2+3+4 wt	38	5′- TGCCGCCGGTCGCCGCCGGTAGGCGGGACTTCCGGTACA -3	++++
NSBE1+2+3 wt	28	5 - TGCCGCCGGTCGCCGCCGGTAGGCGGGAC -3′	++++
NSBE2+3+4	28	5′-AGCCGCCGGTAGGCGGGACTTTCCGGTACA-3	+
NSBE1-3456	28	5 - AGCTATTGGTCGCCGCCGGTAGGCGGGAC -3′	+
NSBE2-3456	28	5′-AGCCGCCGGTCGCTATTGGTAGGCGGGAC-3	+
NSBE3-3456	28	5 -AGCCGCCGGTCGCCGCCGGTAGGTAAAA C-3	++++
NSBE1+2-3456	28	5′-AGCTATTGGTCGCTATTGGTAGGCGGGAC-3′	-
NSBE3-1to8	28	5 - AGCCGCCGGTCGCCGCCGGTATTATTTCA -3′	++++
NSBE1+2	18	5′- TGCCGCCGGTCGCCGCCGG -3	-
NSBE2+3	18	5′- TGCCGCCGGTAGGCGGGAC -3′	-
NSBE3+4	18	5′- AGGCGGGACTTCCGGTACA -3	-
NSBE/19	19	5′-AGCCGCCGGTCGCCGCCGGT-3	-
NSBE/20	20	5 -AGCCGCCGGTCGCCGCCGGTA-3′	-
NSBE/21	21	5′-AGCCGCCGGTCGCCGCCGGTAG-3′	++
NSBE/23	23	5′- AGCCGCCGGTCGCCGCCGGTAGGC -3′	+++
NSBE/25	25	5′- AGCCGCCGGTCGCCGCCGGTAGGCGG-3′	++++
NSBE1+2-1AA	28	5′- AACCGCCGGTCACCGCCGGTAGGCGGGAC-3′	+++
NSBE1+2-2TT	28	5′-AGTCGCCGGTCGTCGCCGGTAGGCGGGAC-3′	++++
NSBE1+2-3TT	28	5′-AGCTGCCGGTCGCTGCCGGTAGGCGGGAC-3′	++++
NSBE1+2-4AA	28	5′-AGCCACCGGTCGCCACCGGTAGGCGGGAC-3′	+++
NSBE1+2-5TT	28	5′-AGCCGTCGGTCGCCGTCGGTAGGCGGGAC-3′	++++
NSBE1+2-6TT	28	5′-AGCCGCTGGTCGCCGCTGGTAGGCGGGAC-3′	+
NSBE1+2-7AA	28	5′-AGCCGCCAGTCGCCGCCAGTAGGCGGGAC-3′	+
NSBE1+2-8AA	28	5′-AGCCGCCGATCGCCGCCGATAGGCGGGAC-3′	+

^*Double-stranded DNA molecules were used in EMSA, but only plus strands of those DNA molecules are listed here. All dsDNA molecules except for the Ori contain a single 5′ overhang on both strands. The bold underlined letters indicate positions of mutation. wt, wild type DNA.

^**The binding affinity of NS1N is shown by plus signs, and the minus sings mean no observed binding.