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. 2014 Mar 13;10(3):e1004221. doi: 10.1371/journal.pgen.1004221

Figure 1. Characterization of natural mum2 mutants.

Figure 1

(A) Schematic representation of the MUM2 gene indicating the positions of the causal mutations in the different accessions and their effect on the encoded protein in capital letters. Boxes represent exons; black shading shows coding sequence; grey shading indicates 5′ and 3′ untranslated regions. (B) Steady state MUM2 mRNA levels in developing siliques of indicated accessions, 8–12 days after pollination, represented as a percentage of the constitutive EF1α-4a (EF) gene abundance. Error bars represent SE (n = 6). (C) Ruthenium red stained seeds of indicated accessions after forced mucilage release by sequential treatment with HCl and NaOH. WT, wild type.