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. 2014 Mar 13;9(3):e90270. doi: 10.1371/journal.pone.0090270

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© 2014 Englund et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Squalene was extracted from wild type and Δshc cultures and the extracts analyzed by HPLC using a squalene standard for identification and quantification of the squalene peak. The identity of the squalene peak was confirmed using GC-MS. (A) HPLC chromatograms (left panels) and GC-MS chromatograms (right panels) for the detection of squalene. A squalene standard (top) was compared with cell extracts from wt (middle) and Δshc mutant (bottom). (B) Fragmentation of squalene standard detected using GC-MS (C) Comparison of squalene content between wild type, Δshc, Δshc:pPMQshc complemented strain, and Δsqs cells. n.d. = no squalene detected.