Skip to main content
. Author manuscript; available in PMC: 2014 Mar 14.
Published in final edited form as: Prostate. 2010 Sep 1;70(12):1274–1285. doi: 10.1002/pros.21163

Fig. 6.

Fig. 6

AIM-100 suppressed androgen-independent pTyr267-AR transcriptional activity and PSA gene expression. A: LAPC4 cells were transfected with the ARR2PB-luciferase reporter. Cells were serum and androgen-depleted (24 hr), treated with DHT (10 nM, 16 hr), and AIM-100 (0.8 μM, 16 hr) and luciferase activity was determined. Data are representative of three independent experiments. B: LAPC4 cells were transfected with the ARR2PB-luciferase reporter. Cells were serum and androgen-depleted (24 hr), treated with EGF (10 ng/ml, 3 hr) and AIM-100 (0.8 μM, 16 hr), and luciferase activity was determined. Data are representative of three independent experiments. *P≤0.008. C: HEK293 cells were transfected with the ARR2PB-luciferase reporter (500 ng), AR vector (500 ng) and caAck1 (500 ng). 24 hr after transfection, cells were treated with AIM-100 (1.6 μM, 16 hr) and luciferase activity was determined. Data are representative of three independent experiments. *P≤0.02. D: HEK293 cells were transfected with the ARR2PB-luciferase reporter (500 ng), AR vector (500 ng), and caAck1 (500 ng). Twenty-four hours after transfection, cells were treated with bicalutamide (1 μM, 16 hr) or flutamide (10 μM, 16 hr), and AIM-100 (1.6 μM, 16 hr) and luciferase activity was determined. Data are representative of three independent experiments. *P≤0.07, **P≤0.05. E: Serum and androgen-depleted LAPC-4 cells were treated with DHT (5 nM, 16 hr), EGF (10 ng/ml, 3 hr), and AM-100 (0.8 μM, 16 hr). Quantitative RT-PCR for PSA mRNA was performed. Data are representative of three similar independent experiments. *P≤0.009; **P≤0.06; ***P≤0.01.