TABLE 1.
Decyder-based alterations in mitochondrial protein expression of KN1ICD in comparison with K562 mock along with their mass spectrometry-based identification and GO annotation for biological functions
Mr, molecular weight.
| Spot no. | Protein name (gene name) | Uniprot accession ID | GO annotation | Average-fold change±S.E. | t Test p value | Theoretical Mr/pI | Mascot score | No of peptides matched | Ion score | % Sequence coverage |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Enoyl-CoA hydratase, mitochondrial (ECHS1) | ECHM_HUMAN | Fatty acid β-oxidation | 1.8 ± 0.074 | 8.40E-06 | 28.32445/5.88 | 116 | 12 | 84 | 37 |
| 2 | Glutaminase kidney isoform, mitochondrial (GLS) | GLSK_HUMAN | Glutamine catabolic process | −1.44 ± 0.0580 | 6.40E-05 | 71.51485/7.01 | 270 | 18 | 207 | 42 |
| 3 | NADH-ubiquinone oxidoreductase 75-kDa subunit, mitochondrial (NDUFS1) | NDUS1_HUMAN | Mitochondrial electron transport, NADH to ubiquinone | −2.63 ± 0.0427 | 0.00018 | 76.92606/5.42 | 273 | 30 | 177 | 38 |
| 4 | NADH-ubiquinone oxidoreductase 75-kDa subunit, mitochondrial (NDUFS1) | NDUS1_HUMAN | Mitochondrial electron transport, NADH to ubiquinone | −1.95 ± 0.0316 | 0.00046 | 76.92606/5.42 | 205 | 27 | 115 | 41 |
| 5 | NADH dehydrogenase (ubiquinone) flavoprotein 2, mitochondrial (NDUFV2) | NDUV2_HUMAN | Mitochondrial electron transport, NADH to ubiquinone | −1.53 ± 0.0583 | 0.00089 | 23.74512/5.71 | 72 | 10 | 25 | 44 |
| 6 | Keratin, type II cytoskeletal 8 (KRT8) | K2C8_HUMAN | Cytoskeleton organization | 3.55 ± 0.5745 | 9.90E-08 | 53.67113/5.52 | 235 | 29 | 83 | 44 |
| 7 | Ornithine aminotransferase, mitochondrial (OAT) | OAT_HUMAN | Cellular amino acid biosynthetic process | −2.07 ± 0.1077 | 0.0014 | 44.78017/5.72 | 454 | 29 | 389 | 52 |
| 8 | Protein disulfide-isomerase A3 (ERP57) | PDIA3_HUMAN | Cell redox homeostasis | 1.68 ± 0.126 | 4.70E-05 | 54.23121/5.61 | 443 | 30 | 310 | 48 |
| 9 | Succinate dehydrogenase (ubiquinone) flavoprotein subunit, mitochondrial (SDHA) | DHSA_HUMAN | Respiratory electron transport chain | −1.56 ± 0.0419 | 3.30E-06 | 67.96871/6.25 | 317 | 33 | 186 | 47 |
| 10 | Dihydrolipoyl dehydrogenase, mitochondrial (DLD) | DLDH_HUMAN | 2-Oxoglutarate metabolic process | 1.49 ± 0.1189 | 0.0004 | 54142.97/7.95 | 378 | 24 | 303 | 39 |
| 11 | Glutamate dehydrogenase 1, mitochondrial (GLUD1) | DHE3_HUMAN | Glutamate catabolic process | −1.44 ± 0.1046 | 0.038 | 61359.19/7.66 | 436 | 24 | 355 | 35 |