FIGURE 7.
Mitochondrial DNA content and analysis of mitochondrial transcription in larvae. All experiments were carried out on w1118, drim2+/−, and drim2−/− larvae. A, mtDNA content was measured by quantitative real time-PCR. Data are ratios of mtDNA to genomic DNA relative to the control ratio in w1118 larvae. Values are expressed as a mean of three independent experiments, and error bars represent the S.D. of the mtDNA/nDNA ratio among the replicates. B, analysis of mitochondrial transcription. Transcripts of the mitochondrial genes COX1 and 16S were measured by real time-PCR and related to the level of transcripts of the nuclear gene Rpl32. For each condition, data are presented as the mean of relative quantity of template in the sample ± S.D. from three individual experiments (Student's t test *, p < 0.05; **, p < 0.01; ***, p < 0.005).