FIGURE 5.

HDAC3 knockdown increases acetylation levels in histones H3 and H4 at the promoters of the CDK6 and NANOS1 genes. A–D, analysis of chromatin levels of HDAC3, H3K9ac, H3K14ac, H4ac, and H3 at the promoter regions of the CDK6 (A and B) and NANOS1 genes (C and D) in H1975 (A and C) and H1792 (B and D) cells. Chromatin levels of proteins and histone marks were measured by quantitative ChIP. Anti-H3 was used as a ChIP control. E, the effect of KDM2A or HDAC3 knockdown on total cellular levels of H3K36me2, H3K9 acetylation, and H4 acetylation. Histone marks were examined by Western blot analysis. H3 and β-actin were used as internal loading controls. Data are presented as the mean ± S.E. (error bars). *, p < 0.05; **, p < 0.01.