TABLE 1.
PCR primers and siRNAs
F, forward; R, reverse.
| Gene | Application | Sequence (5′–3′) |
|---|---|---|
| CDK6 F | RT-qPCR | TCG ATG AAC TAG GCA AAG ACC |
| CDK6 R | RT-qPCR | AGG TGG GAA TCC AGG TTT TC |
| NEK7 F | RT-qPCR | ACT TGG AGA TCT TGG GCT TG |
| NEK7 R | RT-qPCR | GAC AGC CAA GAG ACC AGA TG |
| NANOS1 F | RT-qPCR | GGT CGG CTC GAC ATG GGA CG |
| NANOS1 R | RT-qPCR | CAC ACC CAG CCT TCG CCG TT |
| RAPH1 F | RT-qPCR | GCC AAC TTT TCT TAC CGC TTC |
| RAPH1 R | RT-qPCR | GAT TTG ACG CTT ACT GTT TCC TG |
| β-Actin F | RT-qPCR | GCA CTC TTC CAG CCT TCC |
| β-Actin R | RT-qPCR | TGT CCA CGT CAC ACT TCA TG |
| CDK6 F | ChIP-qPCR | CGG AGA GAG TGC TGG TAA CTC CTT |
| CDK6 R | ChIP-qPCR | TGC GAG TGT CAG TCG GCT CT |
| NEK7 F | ChIP-qPCR | TTC GGC TCC AGT AGG GAA AC |
| NEK7 R | ChIP-qPCR | CTG CCC GAT GGA GGC TT |
| NANOS1 F | ChIP-qPCR | GGA GGA GTG GGC CCG ATA AA |
| NANOS1 R | ChIP-qPCR | AAA GCC TCC ATG GGC GGG |
| RAPH1 F | ChIP-qPCR | CAG TCA GTC AGT CAG TCA GTC AGT |
| RAPH1 R | ChIP-qPCR | AGG GCG AGG CTA ACC ACT CA |
| siLuc | siRNA (Dharmacon) | Sense, 5′-AACTTACGCTGAGTACTTCGA-dTdT-3′ |
| Antisense, 5′-AAUCGAAGUACUCAGCGUAAGUU-3′ | ||
| siKDM2A-3 | siRNA (Dharmacon) | Sense, 5′-AACAAGGAGAGUGUGGUGUUU-dTdT-3′ |
| Antisense, 5′-AAACACCACACUCUCCUUGTT-3′ | ||
| siKDM2A-4 | siRNA (Dharmacon) | Sense, 5′-AAUUACGAAGCCUCACACUAU-dTdT-3′ |
| Antisense, 5′-AUAGUGUGAGGCUUCGUAATT-3′ | ||
| siControl | siRNA (IDT) | Sense, 5′-UCGAAGUACUCAGCGUAAGTT-3′ |
| Antisense, 5′-CUUACGCUGAGUACUUCGATT-3′ | ||
| siHDAC3-3 | siRNA (IDT) | Sense, 5′-CCCGCAUCGAGAAUCAGAACUCACG-3′ |
| Antisense, 5′-CGUGAGUUCUGAUUCUCGAUGCGGGUG-3′ | ||
| siHDAC3-9 | siRNA (IDT) | Sense, 5′-GUCAACGACAUUGUGAUUGGCAUCC-3′ |
| Antisense, 5′-GGAUGCCAAUCACAAUGUCGUUGACAU-3′ | ||
| siCDK6-1 | siRNA (IDT) | Sense, 5′-AGAGUGCAAAUUAGAACUAGAAAGT-3′ |
| Antisense, 5′-ACUUUCUAGUUCUAAUUUGCACUCUAC-3′ | ||
| siCDK6-3 | siRNA (IDT) | Sense, 5′-GGUGUUGUCAGUACUAUAAAUGCTT-3′ |
| Antisense, 5′-AAGCAUUUAUAGUACUGACAACACCUA-3′ | ||
| siNANOS1-1 | siRNA (IDT) | Sense, 5′-GGAGUAGACAGAGUUGUAUGGCCTG-3′ |
| Antisense, 5′-CAGGCCAUACAACUCUGUCUACUCCCA-3′ | ||
| siNANOS1-3 | siRNA (IDT) | Sense, 5′-GGACCACUAUGAACAGAUCAGCGCA-3′ |
| Antisense, 5′-UGCGCUGAUCUGUUCAUAGUGGUCCUU-3′ | ||
| siScramble | siRNA (IDT) | Sense, 5′-GUGUAUGUAGUGCGGUAGUAU-3′ |
| Antisense, 5′-AUACUACCGCACUACAUCAAC-3′ |