Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 5;289(11):7524–7536. doi: 10.1074/jbc.M113.515890

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 10. — Cholesterol-sensitive ubiquitination of ABCA1 and ABCG1. A, control (lanes 1 and 4) and ABCG1-678-expressing (lanes 2, 3, 5, and 6) CHO cells were incubated for 8 h in medium (0.5% (v/v) FBS) containing MG132 (10 μm) with or without cholesterol/CD (20 μg/ml). Whole cell lysates (10 μg protein) were subjected to SDS-PAGE and immunoblotted (IB) for ubiquitin (left panel) or ABCG1 (right panel). B, samples of the same cell lysates were immunoprecipitated (IP) with anti-ABCG1, followed by immunoblotting with an anti-ubiquitin antibody (upper panel). The blots were then reprobed with anti-ABCG1 (lower panel). Molecular weight markers are shown. C and D, results for ABCA1 using ABCA1-CHO (lanes 2, 3, 5, 6, 8, and 9) and control (lanes 1, 4, and 7) cells and conditions as for A and B, except that Western blots for tubulin are also shown in C (lanes 7–9).