FIGURE 6.

Effect of cholesterol loading on other cell proteins. ABCG1-678-CHO cells were incubated for 8 h in medium containing 0.5% (v/v) FBS with or without cholesterol/CD (Chol, 20 μg cholesterol/ml) and with or without cycloheximide (CHX, 10 μg/ml). A–C and F, Na/K-ATPase (A), EEA1 (B), α-tubulin (C), and ABCG1 (F) were measured in cell lysates by Western blot. D and E, in a separate experiment, SR-BI-CHO cells were treated identically and blotted for transferrin receptor (D) and SR-BI (E). The data for each protein are expressed relative to its starting level (T = 0 h) and are means ± S.D. of triplicate determinations from single representative experiments.