FIGURE 7.

Cholesterol loading preserves ABCA1 and ABCG1 protein in human macrophages. THP-1 macrophages were treated for 24 h with T-0901317 (1 μm) and then incubated for 8 h in medium containing cycloheximide (10 μg/ml) with or without cholesterol/CD (20 μg cholesterol/ml). A, cholesterol content at 8 h (mean ± S.D. of three separate experiments). B and D, ABCA1 (B) and ABCG1 (C) levels at 8 h were measured by Western blot. The data are means ± S.D. (n = 3) from a single experiment, representative of four. *, p < 0.05 compared with nonloaded (control) cells. Representative Western blot images are shown. D and E, dose-dependent cholesterol loading (D) and ABCG1 protein levels (E) when THP-1 macrophages were incubated as above with the indicated concentrations of cholesterol. The data are means ± S.D. (n = 3) from a single experiment.