FIGURE 9.

ABCA1 and ABCG1 sensitivity to protease inhibitors. A and B, ABCG1-678 CHO cells (A) or ABCA1-CHO cells (B) were incubated for 8 h in medium (0.5% (v/v) FBS) without (Control) or with (all other conditions) cycloheximide (CHX, 10 μg/ml) together with the inhibitors. Nil, no addition, MG132 (10 μm), ALLN (100 μg/ml), lactacystin (Lact., 10 μm), or chloroquine (Chq., 100 μm). ABCG1 and ABCA1 were measured in cell lysates by Western blotting, and the data are presented relative to the level in untreated control cells. Representative Western blot images are shown. The data are means ± S.D. of triplicate samples from single representative experiments. *, significantly (p < 0.05) different to control condition.