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RSV infection induces the ER stress response. A, quantitative RT-PCR data showing induction of a number of ER stress genes by RSV infection in A549 cells. RNA was isolated from the indicated groups at 24 h post-infection. HPRT, hypoxanthine-guanine phosphoribosyltransferase. B, Western blot analysis showing a time-dependent increase in BiP expression post-RSV infection of A549 cells. Tunicamycin (Tuni) was used at 1 μg/ml for 4 h in both A and B. Cont, control.
