FIGURE 3.

IRE1 inhibits RSV replication. A, Western blot analysis using an all-antigen polyclonal RSV antibody, detecting G, F, P, and M2-1 viral proteins, 8 h post-infection of A549 cells with or without cotreatment with various doses of the IRE1 endonuclease inhibitor 3,5-dibromosalicylaldehide. B, viral titers measured from supernatant of RSV-infected A549 cells with or without cotreatment, as indicated, 8 h post-infection. C, Western blot for RSV proteins performed at different time points on WT versus IRE1 KO MEFs infected with RSV at an MOI of 1 with corresponding densitometry obtained from three independent experiments shown at the bottom. D, viral titers measured from supernatant of RSV-infected wild-type versus IRE1 knockout MEFs 24 h post-infection. E, quantitative RT-PCR data for the G and N RSV genes at 4 and 24 h post-infection of wild-type versus IRE1 knockout MEFs. HPRT, hypoxanthine-guanine phosphoribosyltransferase.