FIGURE 7.

Cell-to-cell fusion mediated by Env at the cell surface. A, cell-free supernatant from donor-transfected HEK293T/17 cells was added to the TZMbl target cells for 6 h in the presence of AZT. Luciferase activity is expressed as RLUs. Data represent one among three separate experiments. B, transfected donor HEK293T/17 cells were co-cultured with TZMbl target cells for 6 h in the presence of AZT. Luciferase activity was normalized to the percentage of FACS-stained Env expressed at the cell surface and expressed as RLU/μg of protein in the cell lysate. Data represent one among three separate experiments.