TABLE 1.

Characteristics and NC50 of the broadly neutralizing antibodies with WT or LLP2_Lys2-mutated viruses

Targeted protein	Antibody	Type of antibody	Targeted epitope	NC50 (ng/μl)a
				WT	LLP2Lys2
gp41	2F5	Monoclonal	Membrane proximal external region	5.3 ± 0.6	5.9 ± 1.1
	4E10	Monoclonal	Membrane proximal external region	6.3 ± 2.9	4.5 ± 2.07
gp120	F425B4e8	Monoclonal	V3 loop	1.9 ± 0.9	1.96 ± 0.6
	2G12	Monoclonal	gp120 carbohydrates	0.15 ± 0.08	0.08 ± 0.002
	HIV Ig	Polyclonal	NAb	206.8 ± 106.7	184.9 ± 110.2
	IgG1b12	Monoclonal	CD4 binding site	0.7 ± 0.1	0.8 ± 0.4
	PGT128	Monoclonal	gp120 carbohydrates	0.02 ± 0.005	0.013 ± 0.004
	Human IgG1 K	Monoclonal	No binding

^a The NC50 were calculated from the standard neutralization assay experiments.

^b NA, data not available.