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Doc H66Y displays reduced toxicity and mRNA stabilization compared with wild type Doc. A, growth curves of E. coli BW25113 cells expressing wild type Doc (■), Doc H66Y (▴), or empty vector (♦). B, Northern blot analysis of the ompA transcript from E. coli total RNA prepared from cells expressing either empty vector (−Doc), +Doc, or +Doc H66Y for the times shown (in minutes) relative to the corresponding uninduced controls (left).
