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Doc phosphorylates recombinant EF-Tu. A, recombinant EF-Tu was combined with wild type (WT) or mutant (H66Y) Doc in a reaction buffer containing [γ-32P]ATP as indicated. B, Doc kinase activity was confirmed by release of ADP. Reactions containing [γ-32P]ATP (ATP only) or supplemented with the indicated proteins were analyzed by thin layer chromatography. The migration of ATP and ADP is indicated on the left.
