FIGURE 5.
Addition of EF-Tu rescues protein synthesis in an in vitro translation system. Production of the dihydrofolate reductase protein (∼20 kDa) was assessed via [35S]methionine incorporation. Supplements were added as indicated. Doc toxin was either pretreated with Phd antitoxin for 15 min prior to the start of the translation reactions (Phd Pretreat) or Phd antitoxin was added to the reaction after 1 h 45 min of translation and allowed to incubate with reaction components (Phd Post-treat). After 15 min either water or EF-Tu was added to the mixture, and translation was allowed to continue.