FIGURE 5.

Hck, specifically the p61 isoform, regulates podosome-mediated matrix degradation. A, WT and Hck^−/− BMMs or control shRNA (Ctrlsh), Hcksh1 and Hcksh2 cells were seeded on Alexa Fluor 568-conjugated fibronectin (Alexa-FN)-coated coverslips and incubated overnight. Degradation area per cell was determined and expressed as a percentage of WT BMM or control shRNA cells (n = 3). **, p < 0.01 compared with WT BMMs or control shRNA cells. B, representative image of RAW/LR5 cell expressing GFP-p61Hck DN stained with rhodamine phalloidin demonstrating the localization of p61HckDN to podosomes. The area of matrix degradation was measured and represented as percentage of the control cells shown in the lower left panel. The lower right panel shows the % of cells with podosomes. **, p < 0.01; n.s, non-significant; Ctrl, control.