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. 2014 Feb 6;289(11):7919–7928. doi: 10.1074/jbc.M113.523621

FIGURE 2.

FIGURE 2.

Enhanced activation of OVA-specific CD4+ T-cells by Pyr-OVA. A, CD4+ T-cells were isolated from OT-II mice, and co-cultured with BMDCs in the presence of 2.0 or 20 μg/ml of native OVA or CM-, CE-, MGO-, Pyr-, or AGE-OVA. Concentrations (conc.) of IL-2 in culture supernatants after 24 h and concentrations of IFN-γ and IL-17A in culture supernatants after 72 h were measured by ELISA. *, p < 0.001; **, p < 0.01. The data are representative of two independent experiments. B, carboxyfluorescein succinimidyl ester (CFSE)-stained CD4+ T-cells were co-cultured with BMDCs and stimulated with 2.0 μg/ml of either form of OVA. After 96 h, the carboxyfluorescein diacetate succinimidyl ester intensity of CD4+ T-cells was measured by flow cytometry. C, CD8+ T-cells were isolated from OT-I mice and co-cultured with BMDCs in the presence of 20 or 200 μg/ml of OVA samples. Concentrations of IL-2 in culture supernatants after 24 h were measured by ELISA. The data are representative of three independent experiments.