FIGURE 4.

Enhanced uptake of Pyr-OVA by BMDCs. A, wild-type BMDCs were incubated with 0.5 or 5.0 μg/ml of FITC-conjugated native OVA or AGE-, CM-, CE-, MGO-OVA, or Pyr-OVA3 (see Table 2) for 15 min. B, wild-type BMDCs were incubated with 5.0 μg/ml of FITC-conjugated native OVA or Pyr-OVA1 (see Table 2). DC uptake of the OVA samples was analyzed by flow cytometry. The gray areas represent cells cultured with medium only. C, after the incubation, the cells were treated with anti-EEA1 antibodies to stain early endosome compartments. DC uptake of the samples was analyzed by confocal microscopy.