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Lack of effect of Pyr-OVA and other glycated OVAs on maturation of BMDCs. BMDCs were stimulated with 50 μg/ml of native OVA, AGE-OVA, or Pyr-OVA or with 10 μg/ml of LPS for 18 h. Expression of CD40, CD80, CD86, and MHC class II molecules on the cell surface of stimulated BMDCs was analyzed by flow cytometry. The gray areas represent cells cultured with medium only. The data are representative of three independent experiments.
