FIGURE 2.

Transfected HEK-293T cells express members of the MR protein family. Western blot analysis of uPARAP (A), MR (B), PLA₂R (C), and DEC-205 (D) expression in whole cell lysates from HEK-293T cells 24 h post transfection. In each panel, duplicate samples from separate transfections with each receptor were analyzed in parallel lanes as indicated. A mouse mAb (2h9) against uPARAP (A), a rat mAb against MR (B), a rabbit pAb against PLA₂R (C), or a rat mAb against DEC-205 (D), were used as primary antibodies for detection. In each case, the assay detected a protein with the theoretical molecular mass (∼180 kDa for uPARAP, MR, and PLA₂R; 205 kDa for DEC-205) in cells transfected with the relevant receptor. No endogenous expression of these receptors was noted except for uPARAP, where a very weak band could be detected by mAb 2h9 in non-uPARAP transfected cells (A). Although the polyclonal anti-PLA₂R antibody gave rise to several unspecific bands (C), these were clearly distinguishable from the specific PLA₂R signal around 180 kDa (marked by black arrowhead).