FIGURE 5.

Ligands internalized by each receptor are degraded lysosomally. The intracellular accumulation of radiolabeled collagen type I (100 ng/ml) in HEK-293T cells transfected with uPARAP (A), MR (B), PLA₂R (C, left panel), and DEC-205 (D, left panel) is shown in the absence or presence of lysosomal protease inhibitor E64d (20 μm). Each sample set includes mock transfected cells for comparison. For PLA₂R (C) and DEC-205 (D), control ligands were also included (100 ng/ml Pancreatic PLA₂ and anti-DEC-205 mAb, respectively; right panels). All other experimental conditions and data presentation were as described for Fig. 3.