FIGURE 9.
A critical FN-II domain loop from uPARAP reconstitutes collagen internalization function in uPARAP chimeras with PLA2R and DEC-205 FN-II domains. A, internalization of radiolabeled collagen type I (100 ng/ml) by HEK-293T cells transfected with uPARAP FN-II chimeras modified to re-introduce the protruding loop from uPARAP (uPARAP-PFNII-Uloop and uPARAP-DFNII-Uloop). Experimental conditions and data presentation were as described for Fig. 6. B, Western blot analysis of the HEK-293T cells expressing the receptor chimeras shown in A. Anti-uPARAP mAb 2h9 was used as primary antibody for detection. Experimental conditions were as described in Fig. 2. Note the weak expression of uPARAP-DFNII-Uloop, which is still above background expression of uPARAP in mock-transfected cells.