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. 2014 Mar 13;9(3):e91172. doi: 10.1371/journal.pone.0091172

Figure 3. Modulation of podocyte motility through shRNAs to non-coding RNAs at the BAIAP2 locus.

Figure 3

A) The number of cells (average +/− SEM) that migrated into the scratch after 48 hours for each of the four conditions: scrambled shRNAs (negative control), shRNAs directed at coding sequence (positive control), antisense shRNAs, and lincRNA shRNAs. As previously shown, shRNA to Baiap2 (coding shRNA) inhibited podocyte migration (p<0.01). shRNAs to the lincRNA further reduced podocyte migration (p<0.001 compared to scrambled shRNA, p<0.01 compared to coding shRNA), while shRNAs to the antisense transcript resulted in increased podocyte motility (p<0.01). (B–D) Phalloidin (actin) stained podocytes treated with B) scrambled shRNAs (normal podocyte appearance), C) antisense shRNAs and D) lincRNA shRNAs. Treatment with shRNAs to the antisense (C) resulted in increased filapodia formation (white arrows), consistent with increased BAIAP2 activity. Treatment with lincRNA shRNAs (D) resulted in an abnormal appearance of the podocytes and the actin cytoskeleton.