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. 2014 Mar 13;9(3):e91337. doi: 10.1371/journal.pone.0091337

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© 2014 Pentzold et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The pH of L. corniculatus leaf macerates is slightly acidic (5.9±0.1 SD, N = 10, green dotted line), whereas the pH measured in the midgut lumen of Z. filipendulae larvae is highly alkaline (10.6±0.1 SD, N = 11, blue dotted line). HCN emission from leaf disc macerates is highest at pH 5–6, which matches the pH of L. corniculatus leaf macerates. However, HCN emission is significantly reduced under highly alkaline conditions at pH 10–11 present in the midgut lumen of Z. filipendulae larvae (one-tailed Student’s t-test, P<0.001). Each data point represents the mean (±SE) of ten independent incubations, i.e. 90 leaf discs were analysed in total.