Table 1.

General features of the T3SS and T4SS primer sequences.

XAC ID	Gene	Gene ID	Product (NCBI Annotation)	Primers (Forward)	Primers (Reverse)	Amplicon (bp)	System
XAC0293	hrpB	1154364	ATP-dependent RNA helicase	GCCGTATCCCGTTGACCTT	ACCGCCCCTTCATCTCTTTT	81	T3SS
XAC0393	hpaF	1154464	HpaF protein	GGCGAGCGTTTTGACAA	CCGGAGATCCTGGCAGTTT	57	T3SS
XAC0394	hrpF	1154465	HrpF protein	GCCGCGACGCAGTTG	CGGTGATCGAGTTGTTCAA	62	T3SS
XAC0396	hpaB	1154467	HpaB protein	GTCCGTGTACGCGCAAGAC	TGACGCCGGCATCATTG	53	T3SS
XAC0397	hrpE	1154468	HrpE protein	ACGAGGCCCAGAAGTCCAT	CCACGTTGAAGTCCAGATCATTT	66	T3SS
XAC0398	hrpD6	1154469	HrpD6 protein	ACCGATACGGTCACCCAAGA	GATCCGCCGCCTTGGT	55	T3SS
XAC0399	hrpD5	1154470	HrpD5 protein	GCCCTGAAGTGCGTTCGTTA	TCACGGACGGGTCTTGCT	54	T3SS
XAC0400	hpaA	1154471	HpaA protein	CGCCACCCCAACAGAAAA	ACGGCATCAGCTCCATCAA	59	T3SS
XAC0401	hrcS	1154472	HrcS protein	CGACGATCTAGTGCGATTTACCT	CGACCACCGGCAAGGA	68	T3SS
XAC0402	hrcR	1154473	Protein T3SS	ACACGGGAACGCGAAAAA	CCTTGGGCCAGATCTGTTGT	58	T3SS
XAC0403	hrcQ	1154474	HrcQ protein	GCAGCTGGAAGTGGACCAA	GGCTGCAAACCCGACAAC	57	T3SS
XAC0404	hpaP	1154475	HpaP protein	TGTCGATGGCACGAAATACC	CCCAACCGCGCCATT	58	T3SS
XAC0405	hrcV	1154476	HrcV protein	GCCGCAAGTTGGTCGAGTT	CTCTGCCATATCGCGATTCC	58	T3SS
XAC0406	hrcU	1154477	HrcU protein T3SS	AGCCGCGGTGCTCTTTG	TAGTCGCCGGCCGATTT	51	T3SS
XAC0407	hrpB1	1154478	HrpB1 protein	CTGCGGCCAGAGCTGAAG	ACCGCGCTTGATGGAAATC	60	T3SS
XAC0408	hrpB2	1154479	HrpB2 protein	CAGCGCAGCAGATCAAGTTG	CGCCGACGCTGACATTG	69	T3SS
XAC0409	hrcJ	1154480	HrcJ protein	GAGCGGCACGCCAATC	TGTCGAGATCAAGCCATCCTT	54	T3SS
XAC0410	hrpB4	1154481	HrpB4 protein	GGACAACACGCGGATCGA	GTCCGTCCAGCGCTGAA	52	T3SS
XAC0411	hrpB5	1154482	Protein T3SS HrpB	CGCACGCCTGGGATATG	AGGCCGGATTCGTTCCA	63	T3SS
XAC0412	hrcN	1154483	T3SS ATPase protein	TCGATGGGCACCTGATTCTC	CGTCGATTGCCGGGTACT	63	T3SS
XAC0413	hrpB7	1154484	HrpB7 protein	CCGCGCTGATGGACAAG	GCTGCAGGATCGTGTCGATA	60	T3SS
XAC0414	hrcT	1154485	HrcT protein	ATCCAGCAATCCGACAGCAT	CACCGGTGCGGACAGTTT	54	T3SS
XAC0415	hrcC	1154486	HrcC protein	CAGATGCGGCTGGATGTG	CCGTCCACGGTGTTGGAT	59	T3SS
XAC0416	hpa1	1154487	Hpa1 protein	GCAGCAGGCCGGTCAGT	TTCATCAGCATCTGGGTGTATTG	57	T3SS
XAC0459	phaE	1154530	monovalent cation/H + antiporter subunit E	CCATCCCGGCTTCATCTG	GCGATGCCGTGAATGTTG	54	T3SS
XAC0618	hrpM	1154689	Glucosyltransferase MdoH	CAAGGGCCTGCACTGGAT	GCATCAACAGACCCCACATC	86	T3SS
XAC1265	hrpG	1155336	HrpG protein	CGGCAAGCCGATCGTATT	GAAAAGAGCAGCCAGGCAAT	54	T3SS
XAC1266	hrpXct	1155337	HrpX protein	GCCTACAGCTACATGATCACCAAT	TGCGGCCACTTCGTTGA	63	T3SS
XAC1520	hrcA	1155591	Heat-inducible transcriptional repressor	AGTTCGCGTTTCGGCATATC	CGGTTCTGCACCTCGTTGT	91	T3SS
XAC1994	hrpX	1156064	HrpX-like protein	CTGGTCTGATGCGAGCTTTCT	GCGTACTCGTCCACGATCAA	90	T3SS
XAC2047	phaE	1156118	PHA synthase subunit	AGCAGGGTGCATCGAAGAAG	TTTTCGGAGCCGCCTTTT	63	T3SS
XAC2606	—	1156677	Hypothetical protein	CCCTCAGTGTGGGCCAGAT	AGGAGGCGGTTGAGGGATA	58	H_T4SS_C
XAC2611	—	1156682	Hypothetical protein	GGGAGCCCGCAATGTG	GGAGGCGTGGGAATATCATCT	59	H_T4SS_C
XAC2612	virB6	1156683	VirB6 protein	TGCAGCAGGGAGGATTAGGA	CGGCGGTGCGGTGAT	56	T4SS_C
XAC2613	—	1156684	Hypothetical protein	CGAAGGGAGATGGAAGCAGTT	GCTCAACAGATCGGCTGGAA	59	H_T4SS_C
XAC2614	virB4	1156685	VirB4 protein	GACGTCGCTTGGTGGAGAAA	TTCCTCGGCGCGAATCT	53	T4SS_C
XAC2615	virB3	1156686	VirB3 protein	GCCCCGCCATGTTTTTG	CGCCCGCACCAATGAA	54	T4SS_C
XAC2616	virB2	1156687	VirB2 protein	TCGCCGATGCCAAAATG	CAGCAACGAAATGAATAGCAATG	62	T4SS_C
XAC2617	virB1	1156688	VirB1 protein	CGTATCGAGTCGTCGCGTAAT	CACCAGGCGTCCACCAA	57	T4SS_C
XAC2618	virB11	1156689	VirB11 protein	CGCTGGTCAACCACATTCC	CCTGGCGTCCTCAATCGT	56	T4SS_C
XAC2619	virB10	1156690	VirB10 protein	CCATTGCAGCTCTCTTGATTCTC	GAATCCTCCCCGCTTTGC	61	T4SS_C
XAC2620	virB9	1156691	VirB9 protein	GCTGAGCCCGAACGAAAAA	GCTCCCATCCACCAGTGAA	59	T4SS_C
XAC2621	virB8	1156692	VirB8 protein	TCGTCATGGCAGATGCGTAT	CCGAAGTTGGGCTCAAGCT	61	T4SS_C
XAC2622	—	1156693	Hypothetical protein	GGCGGATTCCAATATGCAGTT	TGGCCCGATCAAGGTGTAG	59	H_T4SS_C
XAC2623	virD4	1156694	VirD4 protein	TCGCTGGAATCCATTGACCTA	TCAAATCCGAAACGCGAAAT	59	T4SS_C
XAC2922	hrpW	1156993	HrpW protein	GGCGGACACACCGACATC	TGCCTTTCAGGGTGGAGTCTT	58	T3SS
XAC3122	hrpA	1157193	ATP-dependent RNA helicase	TGCGCGCTCTGAATTCG	GGACTGGGTAAGATCTTCATGCA	76	T3SS
XACb0035	—	1158522	Hypothetical protein	CGAGACGAGAACGGCCATT	TGTCACGCAGCAATTCGAA	55	H_T4SS_P
XACb0036	virB1	1158523	VirB1 protein	CGTTACAACCTCGCCAAATATG	CTGCCCGCCTTGAGATTG	74	T4SS_P
XACb0037	virB11	1158524	VirB11 protein	CCCGGAAGCGGTTGAGT	CGTGAGCGACCCCTTGTG	58	T4SS_P
XACb0038	virB10	1158525	VirB10 protein	TCTACGTGAACAAAGACCTCGATT	CGCTTGGCTCCGTCGAT	64	T4SS_P
XACb0039	virB9	1158526	VirB9 protein	GCTCACGCGGCGAAGTT	GCACTTGCTTGACACGATCATC	58	T4SS_P
XACb0040	virB8	1158527	VirB8 protein	CTCGAACAAGCTCAAACAGGAA	AACAGCAGCAACCCGATGA	60	T4SS_P
XACb0041	virB6	1158528	VirB6 protein	GCGCAGCTCGTCATCGT	CGCGGACACCCAACATG	55	T4SS_P
XACb0042	—	1158529	Hypothetical protein	CCGGGAACCCGCTTTTA	GGAAGCCACTGCCGAAACT	52	H_T4SS_P
XACb0043	—	1158530	Hypothetical protein	TTCGCGGCCCTCATCTC	AGGGCGACTTGTTGAACTTGTT	58	H_T4SS_P
XACb0044	virB5	1158531	VirB5 protein	CAGGCCGCCTACGAGAAG	GGGATTGGCGAACAGGTCTT	57	T4SS_P
XACb0045	virB4	1158532	VirB4 protein	GGAACAGGCAAAAGACGTCAAC	CCCGACAAGGTTTGAACGA	57	T4SS_P
XACb0046	virB3	1158533	VirB3 protein	TCTTGCGATGAGCTTTTATTTCC	CCCAAAGTGATCGCGTGATT	59	T4SS_P
XACb0047	virB2	1158534	VirB2 protein	CGATCTGGCGGCTAACGT	ATGCGAGCTGCGGAAGAA	56	T4SS_P
XACb0048	—	1158535	Hypothetical protein	GGCGGATGACGTTGAAGCT	CGCGGTAACGACCTCATAAAC	56	H_T4SS_P
XACb0049	—	1158536	Hypothetical protein	ACCACGGATCCTGGCAAAT	AGCAGCCCCCGTGAACA	51	H_T4SS_P

This table provides primers sequences, ORF's identification ID, gene's name, and respective Xanthomonas citri subsp. citri secretory systems. T4SS: type IV secretion system; T3SS: type III secretion system; C: chromosomal copy; P: plasmid copy; H: hypothetical genes associated with the T4SS.