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. 2014 Mar 13;9(3):e91471. doi: 10.1371/journal.pone.0091471

Table 1. Phenotypes caused by ectopic expression of various forms of dSNAP-29.

Gal4 lines Expression pattern UAS-dSNAP29 UAS-dSNAP29ΔC UAS-dSNAP29RNAi
actin5-Gal4 ubiquitous 1st instar lethal lethal pupal lethal at 29 °C
da-Gal4 ubiquitous 1st instar lethal lethal n.d.
C96-Gal4 wing margin none Notched wings* none
24B-Gal4 muscle (late) none n.d. pupal lethal at 29 °C
mhc-Gal4 muscle (early) none n.d. none
elav-Gal4 CNS none Abnormal wings* none

* Not observed in all the independent UAS lines that were tested.

For each UAS construct, several independent lines were tested to rule out positional effect. Visible phenotypes or sterility were scored. The crosses are set at both room temperature and 29 °C unless indicated otherwise. n.d., not determined.