Table 1. Phenotypes caused by ectopic expression of various forms of dSNAP-29.
Gal4 lines | Expression pattern | UAS-dSNAP29 | UAS-dSNAP29ΔC | UAS-dSNAP29RNAi |
actin5-Gal4 | ubiquitous | 1st instar lethal | lethal | pupal lethal at 29 °C |
da-Gal4 | ubiquitous | 1st instar lethal | lethal | n.d. |
C96-Gal4 | wing margin | none | Notched wings* | none |
24B-Gal4 | muscle (late) | none | n.d. | pupal lethal at 29 °C |
mhc-Gal4 | muscle (early) | none | n.d. | none |
elav-Gal4 | CNS | none | Abnormal wings* | none |
* Not observed in all the independent UAS lines that were tested.
For each UAS construct, several independent lines were tested to rule out positional effect. Visible phenotypes or sterility were scored. The crosses are set at both room temperature and 29 °C unless indicated otherwise. n.d., not determined.