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. 2014 Mar 13;10(3):e1003939. doi: 10.1371/journal.ppat.1003939

Figure 7. LasB cleavage sites in VE-cadherin extracellular domain.

Figure 7

(A) Purified VE-cadherin extracellular domain was incubated with LasB as in Fig. 6 and the N-terminal sequences of protein present in the 50 kDa band were determined. Two sequences were obtained as indicated, both belonging to VE-cadherin sequence. N-terminal positions in VE-cadherin sequence are indicated. (B) Representation of LasB cleavage sites on 2 VE-cadherin molecules located on 2 adjacent endothelial cells. VE-cadherin extracellular domain is constituted by 5 cadherin domains numbered 1-5 from the N-terminus. VE-cadherin homotypic adhesion is promoted by domain 1. LasB cleaves at 2 close positions in the middle of domain 3, precluding endothelial homophilic adhesion.