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. 2014 Mar 13;10(3):e1004003. doi: 10.1371/journal.ppat.1004003

Figure 2. Host cell-secreted TNFα is required for parasite-induced motility.

Figure 2

A) Quantification of TNFα depletion in TaC12 and Thei culture supernatants by ELISA, 48 h after siControl or siTNFα transfections. Red line: detection limit (0.078 ng/ml). B) Paths of individual siControl or siTNFα-transfected TaC12 cells migrating for 8 h. C) Quantifications of path lengths of control and TNFα-depleted TaC12 and Thei cells. Box plots with means of path lengths are shown (TaC12 n = 88, 95, 105 and Thei n = 35, 35, 35) D) Matrigel invasion assay: Bright field microscopy images (100× magnification) of either siControl or siTNFα_1 or siTNFα_2 transfected cells after transmigration. E) Quantification of secreted TNFα dependent transmigration in matrigel assay after 24 h. See also Figure S2.