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PAFAH1b3 is a novel palmitoylated protein regulated by insulin. A, Top-Biotin switch assay for palmitoylation of endogenous PAFAH1b3 protein. Bottom-Effects of insulin on total protein abundance. B, Effects of insulin on palmitoylation of tagged PAFAH1b3. C, The quantification of biotin switch assay for palmitoylation of HA-PAFAH1b3 protein in transfected cells treated with control or insulin (3 independent experiments). * p<0.05 by two-way ANOVA.
