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. 2014 Feb 23;2014:591468. doi: 10.1155/2014/591468

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Copyright © 2014 Ji-Fu Wei et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression and purification of Per a 5 in E. coli. (a) Per a 5 was induced by 0.1, 0.5, and 1 mM IPTG, respectively, and was analyzed by SDS-PAGE. Lane M: Smart Broad-Range Protein Standard (Genscript, Nanjing, China); lanes 1, 2, 3, and 4 represent lysates of cells induced with 0.5, 0.1, 1.0, or 0 mM IPTG, respectively. The arrow indicates Per a 5 protein. (b) SDS-PAGE analysis of purified Per a 5 expressed in E. coli. Lane M: protein standard; lane 1: the supernatant of cells lysate after centrifugation; lane 2: run through; lane 3: wash with 50 mM imidazole; lane 4: wash with 250 mM imidazole. The arrow indicates recombinant Per a 5 protein.